Products

Immunofluorescence

Immunofluorescent staining of Jurkat cells using ITGAL recombinant monoclonal antibody, clone MHM24 (Cat # RAB03154).
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells stained with the chimeric mouse IgG version of RAB03154 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03154- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Inhibition Assay

Flow Cytometry

Flow cytometric analysis of Jurkat cells with ITGAL recombinant monoclonal antibody, clone MHM24 (Cat # RAB03154).
Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of RAB03154 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

3683

Gene Name

ITGAL

Gene Alias

CD11A, LFA-1, LFA1A

Gene Description

integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)

Omim ID

153370

Gene Ontology

Hyperlink

Gene Summary

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

LFA-1 alpha|antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide|integrin alpha L|integrin gene promoter|lymphocyte function-associated antigen 1