SEMA4D recombinant monoclonal antibody, clone 21E8
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More Files
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Flow Cytometry
Flow cytometry analysis of untransfected HEK293T cells (green line) and transfected human SEMA4D HEK293T stable cells (red line) stained with SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated anti-Human IgG Fc antibody.
The ELISA results show that the binding activity of human SEMA4D with SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548). Immobilized human SEMA4D (CSB-MP835707HU) at 2 ug/mL can bind SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548), the EC50 is 1.082-4.805 ng/mL.
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Specification
Product Description
Human recombinant monoclonal antibody raised against human SEMA4D.
Antibody Species
Human
Immunogen
Original antibody is raised against recombinant protein of human SEMA4D.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG4(S228P)
Quality Control Testing
Binding activity
The ELISA results show that the binding activity of human SEMA4D with SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548). Immobilized human SEMA4D (CSB-MP835707HU) at 2 ug/mL can bind SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548), the EC50 is 1.082-4.805 ng/mL.
Recommend Usage
ELISA
Flow Cytometry(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (0.03% Proclin 300 and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of untransfected HEK293T cells (green line) and transfected human SEMA4D HEK293T stable cells (red line) stained with SEMA4D recombinant monoclonal antibody, clone 21E8 (Cat # RAB07548)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated anti-Human IgG Fc antibody. -
Gene Info — SEMA4D
Entrez GeneID
10507Protein Accession#
Q92854Gene Name
SEMA4D
Gene Alias
C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, SEMAJ, coll-4
Gene Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Omim ID
601866Gene Ontology
HyperlinkGene Summary
sema domain
Other Designations
OTTHUMP00000021622|OTTHUMP00000070035|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D|semaphorin 4D
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Interactome
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Pathway
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Disease
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