SEMA4D recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SEMA4D.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human SEMA4D.
Theoretical MW (kDa)
120
Reactivity
Human
Specificity
Recognizes endogenous levels of CD100 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A) whole cell lysates with SEMA4D recombinant monoclonal antibody (Cat # RAB02539).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of Jurkat cells with SEMA4D recombinant monoclonal antibody (Cat # RAB02539). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — SEMA4D
Entrez GeneID
10507Protein Accession#
Q92854Gene Name
SEMA4D
Gene Alias
C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, SEMAJ, coll-4
Gene Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Omim ID
601866Gene Ontology
HyperlinkGene Summary
sema domain
Other Designations
OTTHUMP00000021622|OTTHUMP00000070035|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D|semaphorin 4D
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Interactome
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Pathway
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Disease
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