Products

Western Blot (Cell lysate)

Western blot analysis of K562 whole cell lysate, 293 whole cell lysate with CASP2 recombinant monoclonal antibody, clone 3D4 (Cat # RAB07513).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human lung cancer using CASP2 recombinant monoclonal antibody, clone 3D4 (Cat # RAB07513) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

835

Protein Accession#

P42575

Gene Name

CASP2

Gene Alias

CASP-2, ICH-1L, ICH-1L/1S, ICH1, NEDD2

Gene Description

caspase 2, apoptosis-related cysteine peptidase

Omim ID

600639

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq

Other Designations

ICH-1 protease|NEDD2 apoptosis regulatory|caspase 2|neural precursor cell expressed, developmentally down-regulated 2