ID1 recombinant monoclonal antibody, clone 2C7
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More Files
- More Functions
Western Blot
Western blot analysis of 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate with ID1 recombinant monoclonal antibody, clone 2C7 (Cat # RAB07504).
Immunofluorescence
Immunofluorescent staining of Hela Cells with ID1 recombinant monoclonal antibody, clone 2C7 (Cat # RAB07504). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ID1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ID1.
Theoretical MW (kDa)
Calculated MW: 17, 1
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate with ID1 recombinant monoclonal antibody, clone 2C7 (Cat # RAB07504).Immunofluorescence
Immunofluorescent staining of Hela Cells with ID1 recombinant monoclonal antibody, clone 2C7 (Cat # RAB07504). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ID1
Entrez GeneID
3397Protein Accession#
P41134Gene Name
ID1
Gene Alias
ID, bHLHb24
Gene Description
inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Omim ID
600349Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DNA-binding protein inhibitor ID-1|OTTHUMP00000030534|OTTHUMP00000030535|dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein)|inhibitor of DNA binding 1|inhibitor of differentiation 1
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Interactome
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Pathway
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