Products

Western Blot

Western blot analysis of NIH/3T3 whole cell lysate, HL-60 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate with OGT recombinant monoclonal antibody, clone 8G7 (Cat # RAB07396).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human lung cancer using OGT recombinant monoclonal antibody, clone 8G7 (Cat # RAB07396) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

8473

Protein Accession#

O15294

Gene Name

OGT

Gene Alias

FLJ23071, HRNT1, MGC22921, O-GLCNAC

Gene Description

O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)

Omim ID

300255

Gene Ontology

Hyperlink

Gene Summary

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq

Other Designations

O-GlcNAc transferase p110 subunit|O-linked GlcNAc transferase|OTTHUMP00000032154|OTTHUMP00000032166|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase