OGT rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human OGT peptide using ARM Technology.
Immunogen
A synthetic peptide of human OGT is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human OGT peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — OGT
Entrez GeneID
8473GeneBank Accession#
OGTGene Name
OGT
Gene Alias
FLJ23071, HRNT1, MGC22921, O-GLCNAC
Gene Description
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Omim ID
300255Gene Ontology
HyperlinkGene Summary
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
O-GlcNAc transferase p110 subunit|O-linked GlcNAc transferase|OTTHUMP00000032154|OTTHUMP00000032166|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
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