OGT monoclonal antibody, clone AEDG-15
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human OGT.
Immunogen
A synthetic peptide corresponding to human OGT.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat OGT, in native form and recombinant. Superfamily members of OGT are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of A549 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — OGT
Entrez GeneID
8473Protein Accession#
O15294Gene Name
OGT
Gene Alias
FLJ23071, HRNT1, MGC22921, O-GLCNAC
Gene Description
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Omim ID
300255Gene Ontology
HyperlinkGene Summary
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
O-GlcNAc transferase p110 subunit|O-linked GlcNAc transferase|OTTHUMP00000032154|OTTHUMP00000032166|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
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Interactome
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Pathway
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Disease
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