NCL (phospho T84) recombinant monoclonal antibody, clone R06-4D0
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of U251MG, HepG2, Hela lysates with NCL (phospho T84) recombinant monoclonal antibody, clone R06-4D0 (Cat # RAB04918).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NCL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr84 of human NCL.
Theoretical MW (kDa)
Calculated MW: 77 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U251MG, HepG2, Hela lysates with NCL (phospho T84) recombinant monoclonal antibody, clone R06-4D0 (Cat # RAB04918). -
Gene Info — NCL
Entrez GeneID
4691Protein Accession#
P19338Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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