NCL recombinant monoclonal antibody, clone R08-6H3
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More Files
- More Functions
Western Blot
Western blot analysis of Nucleolin in Hela, A549, HL-60 lysates using Nucleolin antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NCL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human NCL.
Theoretical MW (kDa)
Calculated MW: 77 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Nucleolin in Hela, A549, HL-60 lysates using Nucleolin antibody.Immunoprecipitation
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Gene Info — NCL
Entrez GeneID
4691Protein Accession#
P19338Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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