TCP1 recombinant monoclonal antibody, clone R06-8G2
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More Files
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Immunocytochemistry
Immunocytochemistry staining of HeLa cells with TCP1 recombinant monoclonal antibody, clone R06-8G2 (Cat # RAB04437) (green). DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TCP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human TCP1.
Theoretical MW (kDa)
Calculated MW: 60 kD
Reactivity
Hamster, Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with TCP1 recombinant monoclonal antibody, clone R06-8G2 (Cat # RAB04437) (green). DAPI was stained the cell nucleus (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — TCP1
Entrez GeneID
6950Protein Accession#
P17987Gene Name
TCP1
Gene Alias
CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
Gene Description
t-complex 1
Omim ID
186980Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000017529|T-complex protein 1|T-complex protein 1, alpha subunit|tailless complex polypeptide 1
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Interactome
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Disease
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