TCP1 monoclonal antibody (M01), clone 2B2-D6
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More Files
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Western Blot (Cell lysate)
TCP1 monoclonal antibody (M01), clone 2B2-D6 Western Blot analysis of TCP1 expression in Hela ( Cat # L013V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TCP1 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to TCP1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (87.01 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TCP1.
Immunogen
TCP1 (AAH00665, 1 a.a. ~ 556 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSFGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (87.01 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TCP1 monoclonal antibody (M01), clone 2B2-D6 Western Blot analysis of TCP1 expression in Hela ( Cat # L013V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TCP1 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TCP1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — TCP1
Entrez GeneID
6950GeneBank Accession#
BC000665Protein Accession#
AAH00665Gene Name
TCP1
Gene Alias
CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
Gene Description
t-complex 1
Omim ID
186980Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000017529|T-complex protein 1|T-complex protein 1, alpha subunit|tailless complex polypeptide 1
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Interactome
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Disease
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Publication Reference
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Interaction with the CCT chaperonin complex limits APOBEC3A cytidine deaminase cytotoxicity.
Abby M Green, Rachel A DeWeerd, David R O'Leary, Ava R Hansen, Katharina E Hayer, Katarzyna Kulej, Ariel S Dineen, Julia H Szeto, Benjamin A Garcia, Matthew D Weitzman.
EMBO reports 2021 Sep; 22(9):e52145.
Application：IP, WB-Tr, Human, CEM, HEK 293T, HepaRG, K-562, MDA-MB-231, Ramos, U-2 OS cells.
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TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A.
Pines A, Dijk M, Makowski M, Meulenbroek EM, Vrouwe MG, van der Weegen Y, Baltissen M, French PJ, van Royen ME, Luijsterburg MS, Mullenders LH, Vermeulen M, Vermeulen W, Pannu NS, van Attikum H.
Nature Communications 2018 Mar; 9(1):1040.
Application：WB, Human, VH10-hTert cells.
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BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.
Seo S, Baye LM, Schulz NP, Beck JS, Zhang Q, Slusarski DC, Sheffield VC.
PNAS 2010 Jan; 107(4):1488.
Application：IF, WB-Ti, WB-Tr, Human, Mouse, ARPE-19 cells, Eyes, HEK 293T cells, Testes.
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Interaction with the CCT chaperonin complex limits APOBEC3A cytidine deaminase cytotoxicity.
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