FOXO3 recombinant monoclonal antibody, clone 1E2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FOXO3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FOXO3.
Theoretical MW (kDa)
Calculated MW: 72, 4
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:293 whole cell lysate, Lane 2: Jurkat whole cell lysate, Lane 3: SH-SY5Y whole cell lysate and Lane 4: U87 whole cell lysate with FOXO3 recombinant monoclonal antibody, clone 1E2 (Cat # RAB04380).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with FOXO3 recombinant monoclonal antibody, clone 1E2 (Cat # RAB04380) (diluated at 1:180).Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with FOXO3 recombinant monoclonal antibody, clone 1E2 (Cat # RAB04380) (diluated at 1:180).Immunofluorescence
Immunofluorescent staining of PC3 cells with FOXO3 recombinant monoclonal antibody, clone 1E2 (Cat # RAB04380) (diluated at 1:60). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXO3
Entrez GeneID
2309Protein Accession#
O43524Gene Name
FOXO3
Gene Alias
AF6q21, DKFZp781A0677, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, MGC12739, MGC31925
Gene Description
forkhead box O3
Omim ID
602681Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000016944|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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Interactome
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Pathway
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Disease
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