FOXO3 (phospho S253) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:PC12 whole cell lysate with FOXO3 (phospho S253) recombinant monoclonal antibody (Cat # RAB02766) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using FOXO3 (phospho S253) recombinant monoclonal antibody (Cat # RAB02766). Counter stained with hematoxylin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against FOXO3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant FOXO3.
Theoretical MW (kDa)
71, 97
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of FOXO3A protein only when phosphorylated at Ser253.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:PC12 whole cell lysate with FOXO3 (phospho S253) recombinant monoclonal antibody (Cat # RAB02766) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using FOXO3 (phospho S253) recombinant monoclonal antibody (Cat # RAB02766). Counter stained with hematoxylin. -
Gene Info — FOXO3
Entrez GeneID
2309Protein Accession#
O43524Gene Name
FOXO3
Gene Alias
AF6q21, DKFZp781A0677, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, MGC12739, MGC31925
Gene Description
forkhead box O3
Omim ID
602681Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000016944|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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Interactome
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Pathway
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Disease
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