FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293 whole cell lysate and Lane 3: A549 whole cell lysate with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04226).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04236) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04236) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FOXO3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S253 of human FOXO3.
Theoretical MW (kDa)
Calculated MW: 97 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293 whole cell lysate and Lane 3: A549 whole cell lysate with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04226).Immunohistochemistry
Immunohistochemical staining of human breast cancer with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04236) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human endometrial cancer with FOXO3 (phospho S253) recombinant monoclonal antibody, clone 1E1 (Cat # RAB04236) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXO3
Entrez GeneID
2309Protein Accession#
O43524Gene Name
FOXO3
Gene Alias
AF6q21, DKFZp781A0677, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, MGC12739, MGC31925
Gene Description
forkhead box O3
Omim ID
602681Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000016944|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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Interactome
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Pathway
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Disease
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