ARNT recombinant monoclonal antibody, clone 2F11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate and Lane 4: K562 whole cell lysate with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344).
Immunohistochemistry
Immunohistochemical staining of human prostate cancer with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344) (diluated at 1:183).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344).
Lane 1: rabbit control IgG, Lane 2: RAB04344 precipitates and Lane 3: Input (Hela whole cell lysate).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ARNT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ARNT.
Theoretical MW (kDa)
Calculated MW: 87, 8
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate and Lane 4: K562 whole cell lysate with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344).Immunohistochemistry
Immunohistochemical staining of human prostate cancer with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344) (diluated at 1:183).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with ARNT recombinant monoclonal antibody, clone 2F11 (Cat # RAB04344).
Lane 1: rabbit control IgG, Lane 2: RAB04344 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ARNT
Entrez GeneID
405Protein Accession#
P27540Gene Name
ARNT
Gene Alias
HIF-1beta, HIF1B, HIF1BETA, TANGO, bHLHe2
Gene Description
aryl hydrocarbon receptor nuclear translocator
Omim ID
126110Gene Ontology
HyperlinkGene Summary
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000032943|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com