Products

Western Blot (Cell lysate)

Western blot analysis of Raw264.7 (A), NIH3T3 (B), Hela (C), CHOK1 (D), C6 (E), Ramos (F) whole cell lysates with ARNT recombinant monoclonal antibody (Cat # RAB02591).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using ARNT recombinant monoclonal antibody (Cat # RAB02591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.96). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Entrez GeneID

405

Protein Accession#

P27540

Gene Name

ARNT

Gene Alias

HIF-1beta, HIF1B, HIF1BETA, TANGO, bHLHe2

Gene Description

aryl hydrocarbon receptor nuclear translocator

Omim ID

126110

Gene Ontology

Hyperlink

Gene Summary

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq

Other Designations

OTTHUMP00000032943|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit