TSPO recombinant monoclonal antibody, clone 23G2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: A549 whole cell lysate and Lane 5: mouse kidney tissue with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341).
Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:117)
Immunohistochemistry
Immunohistochemical staining of human colon cancer with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:117)
Immunofluorescence
Immunofluorescent staining of PC3 cells with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:39). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341).
Lane 1: rabbit control IgG, Lane 2: RAB04341 precipitates and Lane 3: Input (Hela whole cell lysate).
Flow Cytometry
Flow cytometric analysis of HepG2 cells with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TSPO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human TSPO.
Theoretical MW (kDa)
Calculated MW: 19, 1
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: A549 whole cell lysate and Lane 5: mouse kidney tissue with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341).Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:117)Immunohistochemistry
Immunohistochemical staining of human colon cancer with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:117)Immunofluorescence
Immunofluorescent staining of PC3 cells with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:39). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341).
Lane 1: rabbit control IgG, Lane 2: RAB04341 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells with TSPO recombinant monoclonal antibody, clone 23G2 (Cat # RAB04341) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — TSPO
Entrez GeneID
706Protein Accession#
P30536Gene Name
TSPO
Gene Alias
BZRP, DBI, IBP, MBR, PBR, PKBS, PTBR, mDRC, pk18
Gene Description
translocator protein (18kDa)
Omim ID
109610Gene Ontology
HyperlinkGene Summary
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000028891|benzodiazepine peripheral binding site|mitochondrial benzodiazepine receptor|peripheral benzodiazepine receptor|peripheral-type benzodiazepine receptor|translocator protein
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Interactome
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Pathway
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Disease
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