TSPO recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:293T whole cell lysate Lane2:NIH/3T3 whole cell lysate Lane3:HepG2 whole cell lysate with TSPO recombinant monoclonal antibody (Cat # RAB02650) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using TSPO recombinant monoclonal antibody (Cat # RAB02650). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of human HeLa cells using TSPO recombinant monoclonal antibody (Cat # RAB02650)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TSPO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant human TSPO.
Theoretical MW (kDa)
18
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of PBR and does not cross-react with related proteins.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:293T whole cell lysate Lane2:NIH/3T3 whole cell lysate Lane3:HepG2 whole cell lysate with TSPO recombinant monoclonal antibody (Cat # RAB02650) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using TSPO recombinant monoclonal antibody (Cat # RAB02650). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of human HeLa cells using TSPO recombinant monoclonal antibody (Cat # RAB02650)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — TSPO
Entrez GeneID
706Protein Accession#
P30536Gene Name
TSPO
Gene Alias
BZRP, DBI, IBP, MBR, PBR, PKBS, PTBR, mDRC, pk18
Gene Description
translocator protein (18kDa)
Omim ID
109610Gene Ontology
HyperlinkGene Summary
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000028891|benzodiazepine peripheral binding site|mitochondrial benzodiazepine receptor|peripheral benzodiazepine receptor|peripheral-type benzodiazepine receptor|translocator protein
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Interactome
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Pathway
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Disease
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