GPC3 recombinant monoclonal antibody, clone 4H11
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate and Lane 2: K562 whole cell lysate with GPC3 recombinant monoclonal antibody, clone 4H11 (Cat # RAB04332).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with GPC3 recombinant monoclonal antibody, clone 4H11 (Cat # RAB04332) (diluated at 1:30). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GPC3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GPC3.
Theoretical MW (kDa)
Calculated MW: 66, 6
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate and Lane 2: K562 whole cell lysate with GPC3 recombinant monoclonal antibody, clone 4H11 (Cat # RAB04332).Immunofluorescence
Immunofluorescent staining of HepG2 cells with GPC3 recombinant monoclonal antibody, clone 4H11 (Cat # RAB04332) (diluated at 1:30). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPC3
Entrez GeneID
2719Protein Accession#
P51654Gene Name
GPC3
Gene Alias
DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1
Gene Description
glypican 3
Gene Ontology
HyperlinkGene Summary
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000024058|OTTHUMP00000062492|glypican proteoglycan 3
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