ACTA1 recombinant monoclonal antibody, clone 25E3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: A549 whole cell lysate, Lane 3: Raw264.7 whole cell lysate, Lane 4: NIH/3T3 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: rat brain tissue and Lane 7: rat heart tissue with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218).
Immunohistochemistry
Immunohistochemical staining of human kidney tissue with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:60). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ACTA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ACTA1.
Theoretical MW (kDa)
Calculated MW: 42 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: A549 whole cell lysate, Lane 3: Raw264.7 whole cell lysate, Lane 4: NIH/3T3 whole cell lysate, Lane 5: HepG2 whole cell lysate, Lane 6: rat brain tissue and Lane 7: rat heart tissue with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218).Immunohistochemistry
Immunohistochemical staining of human kidney tissue with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of HepG2 cells with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:60). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with ACTA1 recombinant monoclonal antibody, clone 25E3 (Cat # RAB04218) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — ACTA1
Entrez GeneID
58Protein Accession#
P68133Gene Name
ACTA1
Gene Alias
ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Description
actin, alpha 1, skeletal muscle
Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
Other Designations
OTTHUMP00000036123|alpha skeletal muscle actin
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Interactome
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Disease
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