ACTA1 recombinant monoclonal antibody, clone R05-4F6
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More Files
- More Functions
Western Blot
Western blot analysis of muscle Actin in Hela, CHO-K1, C6, rat Brain lysates using muscle Actin antibody.
Immunocytochemistry
Immunocytochemistry analysis of alpha Skeletal Muscle Actin (green) in Hela using alpha Skeletal Muscle Actin antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ACTA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human ACTA1.
Theoretical MW (kDa)
Calculated MW: 42 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of muscle Actin in Hela, CHO-K1, C6, rat Brain lysates using muscle Actin antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of alpha Skeletal Muscle Actin (green) in Hela using alpha Skeletal Muscle Actin antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — ACTA1
Entrez GeneID
58Protein Accession#
P68133Gene Name
ACTA1
Gene Alias
ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Description
actin, alpha 1, skeletal muscle
Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
Other Designations
OTTHUMP00000036123|alpha skeletal muscle actin
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Interactome
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Disease
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