MCAM recombinant monoclonal antibody, clone 8A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A375 whole cell lysate with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174).
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of U251 cells with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:37). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of A375 cells with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MCAM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MCAM .
Theoretical MW (kDa)
Calculated MW: 72 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A375 whole cell lysate with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174).Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of U251 cells with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:37). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A375 cells with MCAM recombinant monoclonal antibody, clone 8A10 (Cat # RAB04174) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — MCAM
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Interactome
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