MCAM recombinant monoclonal antibody, clone R05-5A2
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More Files
- More Functions
Western Blot
Western blot analysis of CD146 in C6, Hela lysates using human CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human colon cancer with CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687).
Immunocytochemistry
Immunocytochemical staining of Hela with CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687). (A) CD146 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human CD146.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD146
Theoretical MW (kDa)
Calculated MW: 72 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CD146 in C6, Hela lysates using human CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human colon cancer with CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of Hela with CD146 recombinant monoclonal antibody, clone R05-5A2 (Cat # RAB01687). (A) CD146 (green) and (B) DAPI (blue). -
Gene Info — MCAM
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Interactome
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