F3 recombinant monoclonal antibody, clone 8B10
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HL60 whole cell lysate; Lane 2: U251 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: NTERA-2 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: Jurkat whole cell lysate; Lane 7: Rat brain tissue.
Immunohistochemistry
Immunohistochemistry image of F3 recombinant monoclonal antibody, clone 8B10 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and rat F3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human F3.
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HL60 whole cell lysate; Lane 2: U251 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: NTERA-2 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: Jurkat whole cell lysate; Lane 7: Rat brain tissue.Immunohistochemistry
Immunohistochemistry image of F3 recombinant monoclonal antibody, clone 8B10 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — F3
Entrez GeneID
2152Protein Accession#
P13726Gene Name
F3
Gene Alias
CD142, TF, TFA
Gene Description
coagulation factor III (thromboplastin, tissue factor)
Omim ID
134390Gene Ontology
HyperlinkGene Summary
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq
Other Designations
OTTHUMP00000012426|coagulation factor III|tissue factor
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Interactome
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Pathway
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Disease
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