F3 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Hela (A), A549 (B), MCF7 (C), U251 (D), HL60 (E), mouse brain (F), rat brain (G) whole cell lysates with F3 recombinant monoclonal antibody (Cat # RAB02563).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using F3 recombinant monoclonal antibody (Cat # RAB02563). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.88). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human F3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human F3.
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of CD142 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela (A), A549 (B), MCF7 (C), U251 (D), HL60 (E), mouse brain (F), rat brain (G) whole cell lysates with F3 recombinant monoclonal antibody (Cat # RAB02563).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using F3 recombinant monoclonal antibody (Cat # RAB02563). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.88). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — F3
Entrez GeneID
2152Protein Accession#
P13726Gene Name
F3
Gene Alias
CD142, TF, TFA
Gene Description
coagulation factor III (thromboplastin, tissue factor)
Omim ID
134390Gene Ontology
HyperlinkGene Summary
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq
Other Designations
OTTHUMP00000012426|coagulation factor III|tissue factor
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Interactome
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Pathway
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Disease
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