Cd80 recombinant monoclonal antibody, clone RM80
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More Files
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Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Cd80 recombinant monoclonal antibody, clone RM80 (Cat # RAB03342).
RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03342 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03342- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd80.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
Flow Cytometry
Functional Study
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Cd80 recombinant monoclonal antibody, clone RM80 (Cat # RAB03342).
RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03342 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03342- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Functional Study
Blocking
Flow Cytometry
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Gene Info — Cd80
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