Cd80 monoclonal antibody, clone 1G10 (FITC)
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Specification
Product Description
Rat monoclonal antibody raised against native Cd80.
Immunogen
Native purified Cd80 from dibutyryl cAMP-activated 5C2 cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Mouse CD80 (B7-1), Mr 45-47 KDa
Form
Liquid
Conjugation
FITC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
P815 cells transfected with Cd80 or non-transfected P815 control cells were stained with Cd80 monoclonal antibody, clone 1G10 (FITC) (Cat # MAB5948). Large cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd80
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Publication Reference
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Costimulation of IL-4 production by murine B7-1 and B7-2 molecules.
Natesan M, Razi-Wolf Z, Reiser H.
Journal of Immunology 1996 Apr; 156(8):2783.
Application:Flow Cyt, Mouse, CHO cells.
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Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.
Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ.
The Journal of Experimental Medicine 1994 Aug; 180(2):631.
Application:Flow Cyt, Mouse, Mouse B cells, CHO cells, Macrophages, T cells.
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Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression.
Nabavi N, Freeman GJ, Gault A, Godfrey D, Nadler LM, Glimcher LH.
Nature 1992 Nov; 360(6401):266.
Application:Flow Cyt, Mouse, 5C2 cells.
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Costimulation of IL-4 production by murine B7-1 and B7-2 molecules.
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