CD40LG recombinant monoclonal antibody, clone chi220
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More Files
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Immunofluorescence
Immunofluorescenct staining of Daudi cells with CD40LG recombinant monoclonal antibody, clone chi220 (Cat # RAB03269).
Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03269 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03269- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD40LG recombinant monoclonal antibody, clone chi220 (Cat # RAB03269).
Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03269 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD40LG.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescenct staining of Daudi cells with CD40LG recombinant monoclonal antibody, clone chi220 (Cat # RAB03269).
Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03269 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03269- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Blocking
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD40LG recombinant monoclonal antibody, clone chi220 (Cat # RAB03269).
Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03269 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CD40LG
Entrez GeneID
959Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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Interactome
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Pathway
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Disease
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