CD40LG recombinant monoclonal antibody, clone YMF323.6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD40LG.
Antibody Species
Rabbit
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of human peripheral blood monocytes (PBMs) with CD40LG recombinant monoclonal antibody, clone YMF323.6 (Cat # RAB03164).
Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of RAB03164 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.Flow Cytometry
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Gene Info — CD40LG
Entrez GeneID
959Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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