Products

Immunofluorescence

Immunofluorescent staining of Jurkat cells with CR1 recombinant monoclonal antibody, clone HB-8592 (Cat # RAB03231).
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RAB03231 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03231- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Flow Cytometry

Flow cytometric analysis of Jurkat cells with CR1 recombinant monoclonal antibody, clone HB-8592 (Cat # RAB03231).
Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of RAB03231 (blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

1378

Gene Name

CR1

Gene Alias

C3BR, CD35, KN

Gene Description

complement component (3b/4b) receptor 1 (Knops blood group)

Omim ID

120620 607486 611162

Gene Ontology

Hyperlink

Gene Summary

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq

Other Designations

C3-binding protein|C3b/C4b receptor|CD35 antigen|Knops blood group antigen|complement component receptor 1|complement receptor 1