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Immunohistochemistry

Immunofluorescence

Immunofluorescent staining of Jurkat cells with CD1A recombinant monoclonal antibody, clone CBT6 (Cat # RAB03189).
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03189 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03189- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunoprecipitation

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of Molt4 cellls with CD1A recombinant monoclonal antibody, clone CBT6 (Cat # RAB03189).
Molt4 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03189 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

909

Gene Name

CD1A

Gene Alias

CD1, FCB6, HTA1, R4, T6

Gene Description

CD1a molecule

Omim ID

188370

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq

Other Designations

CD1A antigen|CD1A antigen, a polypeptide|OTTHUMP00000018907|T-cell surface antigen T6/Leu-6|T-cell surface glycoprotein CD1a|cluster of differentiation 1 A|cortical thymocyte antigen CD1A|differentiation antigen CD1-alpha-3|epidermal dendritic cell marker