CCR5 (phospho Serine337) recombinant monoclonal antibody, clone V14/2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CCR5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to C-terminus of human CCR5.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of K562 cell lysate with CCR5 (phospho Serine337) recombinant monoclonal antibody, clone V14/2 (Cat # RAB03131).Immunofluorescence
Immunofluorescent staining of U937 cells with CCR5 (phospho Serine337) recombinant monoclonal antibody, clone V14/2 (Cat # RAB03131).
Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03131 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03131- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of U937 cells with CCR5 (phospho Serine337) recombinant monoclonal antibody, clone V14/2 (Cat # RAB03131).
U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03131 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CCR5
Entrez GeneID
1234Gene Name
CCR5
Gene Alias
CC-CKR-5, CCCKR5, CD195, CKR-5, CKR5, CMKBR5, IDDM22
Gene Description
chemokine (C-C motif) receptor 5
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
C-C chemokine receptor 5|C-C chemokine receptor type 5|CCR5 chemokine receptor|chemokine (C-C) receptor 5|chemokine receptor CCR5|chemr13
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