CBX2 recombinant monoclonal antibody, clone RAB-C238
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More Files
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Immunofluorescence
Immunofluorescent staining of HeLa cells with CBX2 recombinant monoclonal antibody, clone RAB-C238 (Cat # RAB03123).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03123 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03123- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells with CBX2 recombinant monoclonal antibody, clone RAB-C238 (Cat # RAB03123).
HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03123 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CBX2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against CBX2 protein under non-denaturing conditions.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ChIP
ELISA
Flow Cytometry
Immunofluorescence
IP-MS
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Immunofluorescence
Immunofluorescent staining of HeLa cells with CBX2 recombinant monoclonal antibody, clone RAB-C238 (Cat # RAB03123).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03123 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03123- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hela cells with CBX2 recombinant monoclonal antibody, clone RAB-C238 (Cat # RAB03123).
HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03123 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CBX2
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Interactome
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