CBX2 monoclonal antibody, clone 4C11B10
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More Files
- More Functions
Western Blot
Western Blot analysis of human CBX2 recombinant protein.
Western Blot
Western Blot analysis of (1) HUVEC, (2) HEK293, (3) Hela, (4) NIH/3T3, and (5) A431 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) cervical cancer tissues and (B) rectum cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CBX2 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CBX2 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CBX2.
Immunogen
Recombinant protein corresponding to amino acids 402-525 of of human CBX2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
56
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Immunofluorescence (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human CBX2 recombinant protein.Western Blot
Western Blot analysis of (1) HUVEC, (2) HEK293, (3) Hela, (4) NIH/3T3, and (5) A431 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of (A) cervical cancer tissues and (B) rectum cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CBX2 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using CBX2 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — CBX2
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Interactome
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Publication Reference
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Subcellular expression pattern and clinical significance of CBX2 and CBX7 in breast cancer subtypes.
Sungjoon Park, Jaehyuck Choi, Jung-Kook Song, Bogun Jang, Young Hee Maeng
Medical Molecular Morphology 2023 Aug; [Epub]:0.
Application：IHC, Human, Human breast.
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Bioinformatic analysis of the expression and prognostic value of chromobox family proteins in human breast cancer.
Xiaomin Li, Junhe Gou, Hongjiang Li, Xiaoqin Yang.
Scientific Reports 2020 Oct; 10(1):17739.
Application：IHC, Human, Breast cancer tissues, Tumor-adjacent normal tissues.
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Subcellular expression pattern and clinical significance of CBX2 and CBX7 in breast cancer subtypes.
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