CBX1 recombinant monoclonal antibody, clone RAB-C145
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More Files
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Western Blot
Western blot analysis of Lane 1: HeLa, Lane 2: HEK293 and Lane 3: MCF7 cell lysates with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).
Immunofluorescence
Immunofluorescent staining of HeLa cells with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03121 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03121- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).
HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03121 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CBX1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against CBX1 protein under non-denaturing conditions.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ChIP
ELISA
Flow Cytometry
Immunofluorescence
IP-MS
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Western Blot
Western blot analysis of Lane 1: HeLa, Lane 2: HEK293 and Lane 3: MCF7 cell lysates with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).Immunofluorescence
Immunofluorescent staining of HeLa cells with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03121 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03121- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hela cells with CBX1 recombinant monoclonal antibody, clone RAB-C145 (Cat # RAB03121).
HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03121 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CBX1
Entrez GeneID
10951Gene Name
CBX1
Gene Alias
CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta
Gene Description
chromobox homolog 1 (HP1 beta homolog Drosophila )
Omim ID
604511Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
heterochromatin protein 1-beta|heterochromatin protein p25 beta|modifier 1 protein
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Interactome
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