CBX1 monoclonal antibody, clone 5A3
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: COS7, Lane 3: NIH/3T3, Lane 4: A431 and Lane5: C6 cell lysate.
Immunofluorescence
Immunofluorescence staining of HeLa cells using CBX1 monoclonal antibody, clone 5A3 (Green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of COS7 cells using CBX1 monoclonal antibody, clone 5A3 (Green) and negative control (Purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CBX1.
Immunogen
Recombinant protein corresponding to human CBX1.
Host
Mouse
Reactivity
Human, Monkey, Mouse
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: COS7, Lane 3: NIH/3T3, Lane 4: A431 and Lane5: C6 cell lysate.Immunofluorescence
Immunofluorescence staining of HeLa cells using CBX1 monoclonal antibody, clone 5A3 (Green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of COS7 cells using CBX1 monoclonal antibody, clone 5A3 (Green) and negative control (Purple). -
Gene Info — CBX1
Entrez GeneID
10951Protein Accession#
P83916Gene Name
CBX1
Gene Alias
CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta
Gene Description
chromobox homolog 1 (HP1 beta homolog Drosophila )
Omim ID
604511Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
heterochromatin protein 1-beta|heterochromatin protein p25 beta|modifier 1 protein
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