UBB recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The heart tissue lysate of Mouse with UBB recombinant monoclonal antibody (Cat # RAB02749) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using UBB recombinant monoclonal antibody (Cat # RAB02749). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of MCF7 cells using UBB recombinant monoclonal antibody (Cat # RAB02749). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against UBB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant UBB.
Theoretical MW (kDa)
8, uncertain
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The heart tissue lysate of Mouse with UBB recombinant monoclonal antibody (Cat # RAB02749) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using UBB recombinant monoclonal antibody (Cat # RAB02749). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of MCF7 cells using UBB recombinant monoclonal antibody (Cat # RAB02749). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — UBB
Entrez GeneID
7314Protein Accession#
P0CG47;P0CG48;P62979;P62987Gene Name
UBB
Gene Alias
FLJ25987, MGC8385
Gene Description
ubiquitin B
Omim ID
191339Gene Ontology
HyperlinkGene Summary
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000064960|OTTHUMP00000064961|polyubiquitin B
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Interactome
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