Products

Western Blot (Cell lysate)

Western blot analysis of Hela (A), A549 (B) whole cell lysates with UBB recombinant monoclonal antibody (Cat # RAB02617).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using UBB recombinant monoclonal antibody (Cat # RAB02617). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.66). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of HeLa cells with UBB recombinant monoclonal antibody (Cat # RAB02617). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Immunoprecipitation

Entrez GeneID

7314

Protein Accession#

P0CG47

Gene Name

UBB

Gene Alias

FLJ25987, MGC8385

Gene Description

ubiquitin B

Omim ID

191339

Gene Ontology

Hyperlink

Gene Summary

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq

Other Designations

OTTHUMP00000064960|OTTHUMP00000064961|polyubiquitin B