Ubiquitin polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of tissue and cell extracts with Ubiquitin polyclonal antibody (Cat # PAB19486).
Lane 1, rat heart tissue lysate.
Lane 2, rat liver tissue lysate.
Lane 3, MM231 whole cell lysate.
Lane 4, HeLa whole cell lysate.
Lane 5, SMMC whole cell lysate.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ubiquitin.
Immunogen
A synthetic peptide corresponding to amino acids 29-45 at N-terminus of human Ubiquitin.
Sequence
KIQDKEGIPPDQQRLIF
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
It identical to the rat and mouse sequences.
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of tissue and cell extracts with Ubiquitin polyclonal antibody (Cat # PAB19486).
Lane 1, rat heart tissue lysate.
Lane 2, rat liver tissue lysate.
Lane 3, MM231 whole cell lysate.
Lane 4, HeLa whole cell lysate.
Lane 5, SMMC whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — UBB
Entrez GeneID
7314Gene Name
UBB
Gene Alias
FLJ25987, MGC8385
Gene Description
ubiquitin B
Omim ID
191339Gene Ontology
HyperlinkGene Summary
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000064960|OTTHUMP00000064961|polyubiquitin B
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