CALD1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:NIH/3T3 whole cell lysate with CALD1 recombinant monoclonal antibody (Cat # RAB02744) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using CALD1 recombinant monoclonal antibody (Cat # RAB02744).Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of C2C12 cells using CALD1 recombinant monoclonal antibody (Cat # RAB02744)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against CALD1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant CALD1.
Theoretical MW (kDa)
70-80, 120-150
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Caldesmon and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:NIH/3T3 whole cell lysate with CALD1 recombinant monoclonal antibody (Cat # RAB02744) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using CALD1 recombinant monoclonal antibody (Cat # RAB02744).Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of C2C12 cells using CALD1 recombinant monoclonal antibody (Cat # RAB02744)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — CALD1
Entrez GeneID
800Protein Accession#
Q05682Gene Name
CALD1
Gene Alias
CDM, H-CAD, L-CAD, MGC21352, NAG22
Gene Description
caldesmon 1
Omim ID
114213Gene Ontology
HyperlinkGene Summary
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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