DES recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The skeletal muscle lysate of Human Lane2:The heart tissue lysate of Mouse with DES recombinant monoclonal antibody (Cat # RAB02728) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using DES recombinant monoclonal antibody (Cat # RAB02728). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of C2C12 cells using DES recombinant monoclonal antibody (Cat # RAB02728)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against DES.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant DES.
Theoretical MW (kDa)
54
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of Desmin and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The skeletal muscle lysate of Human Lane2:The heart tissue lysate of Mouse with DES recombinant monoclonal antibody (Cat # RAB02728) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using DES recombinant monoclonal antibody (Cat # RAB02728). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of C2C12 cells using DES recombinant monoclonal antibody (Cat # RAB02728)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — DES
Entrez GeneID
1674Protein Accession#
P17661Gene Name
DES
Gene Alias
CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793
Gene Description
desmin
Gene Ontology
HyperlinkGene Summary
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq
Other Designations
OTTHUMP00000064865|intermediate filament protein
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Interactome
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Pathway
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Disease
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