DES polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis with DES polyclonal antibody (Cat # PAB30549)
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver tissue
Lane 5: Human tonsil tissue
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart muscle with DES polyclonal antibody (Cat # PAB30549) shows strong cytoplasmic positivity in myocytes.
Immunofluorescence
Immunofluorescent staining of human cell line RH-30 with DES polyclonal antibody (Cat # PAB30549) shows positivity in intermediate filaments. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human DES.
Immunogen
Recombinant protein corresponding to human DES.
Sequence
KLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis with DES polyclonal antibody (Cat # PAB30549)
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver tissue
Lane 5: Human tonsil tissueImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart muscle with DES polyclonal antibody (Cat # PAB30549) shows strong cytoplasmic positivity in myocytes.Immunofluorescence
Immunofluorescent staining of human cell line RH-30 with DES polyclonal antibody (Cat # PAB30549) shows positivity in intermediate filaments. Antibody staining is shown in green. -
Gene Info — DES
Entrez GeneID
1674Protein Accession#
P17661Gene Name
DES
Gene Alias
CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793
Gene Description
desmin
Gene Ontology
HyperlinkGene Summary
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq
Other Designations
OTTHUMP00000064865|intermediate filament protein
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Interactome
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Pathway
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Disease
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