MCL1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:Raji whole cell lysate Lane2:The heart tissue lysate of Mouse with MCL1 recombinant monoclonal antibody (Cat # RAB02724) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using MCL1 recombinant monoclonal antibody (Cat # RAB02724).Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using MCL1 recombinant monoclonal antibody (Cat # RAB02724)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against MCL1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant MCL1.
Theoretical MW (kDa)
29, 37
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of MCL1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:Raji whole cell lysate Lane2:The heart tissue lysate of Mouse with MCL1 recombinant monoclonal antibody (Cat # RAB02724) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using MCL1 recombinant monoclonal antibody (Cat # RAB02724).Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using MCL1 recombinant monoclonal antibody (Cat # RAB02724)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — MCL1
Entrez GeneID
4170Protein Accession#
Q07820Gene Name
MCL1
Gene Alias
BCL2L3, EAT, MCL1L, MCL1S, MGC104264, MGC1839, TM
Gene Description
myeloid cell leukemia sequence 1 (BCL2-related)
Omim ID
159552Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. [provided by RefSeq
Other Designations
OTTHUMP00000032794|OTTHUMP00000032795|induced myeloid leukemia cell differentiation protein Mcl-1|myeloid cell leukemia sequence 1|myeloid cell leukemia sequence 1, isoform 1
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Interactome
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Disease
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