CBX5 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF7 whole cell lysate Lane2:Hela whole cell lysate with CBX5 recombinant monoclonal antibody (Cat # RAB02715) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using CBX5 recombinant monoclonal antibody (Cat # RAB02715). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of MCF7 cells using CBX5 recombinant monoclonal antibody (Cat # RAB02715). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against CBX5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant CBX5.
Theoretical MW (kDa)
25
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of CBX5 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF7 whole cell lysate Lane2:Hela whole cell lysate with CBX5 recombinant monoclonal antibody (Cat # RAB02715) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using CBX5 recombinant monoclonal antibody (Cat # RAB02715). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of MCF7 cells using CBX5 recombinant monoclonal antibody (Cat # RAB02715). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — CBX5
Entrez GeneID
23468Protein Accession#
P45973Gene Name
CBX5
Gene Alias
HP1, HP1A
Gene Description
chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Omim ID
604478Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
HP1-ALPHA|HP1Hs alpha|antigen p25|heterochromatin protein 1 homolog alpha|heterochromatin protein 1-alpha
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Interactome
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