CBX5 recombinant monoclonal antibody, clone R09-9D5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with CBX5 recombinant monoclonal antibody, clone R09-9D5 (Cat # RAB02206).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with CBX5 recombinant monoclonal antibody, clone R09-9D5 (Cat # RAB02206). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CBX5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CBX5.
Theoretical MW (kDa)
Calculated MW: 22 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with CBX5 recombinant monoclonal antibody, clone R09-9D5 (Cat # RAB02206).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with CBX5 recombinant monoclonal antibody, clone R09-9D5 (Cat # RAB02206). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — CBX5
Entrez GeneID
23468Protein Accession#
P45973Gene Name
CBX5
Gene Alias
HP1, HP1A
Gene Description
chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Omim ID
604478Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
HP1-ALPHA|HP1Hs alpha|antigen p25|heterochromatin protein 1 homolog alpha|heterochromatin protein 1-alpha
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