LGALS3 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LGALS3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human Galectin 3.
Theoretical MW (kDa)
28
Reactivity
Human
Specificity
Recognizes endogenous levels of Galectin 3 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A) whole cell lysates with Galectin 3 recombinant monoclonal antibody (Cat # RAB02502).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using Galectin 3 recombinant monoclonal antibody (Cat # RAB02502). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.58). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of Galectin 3 staining in U87MG cells using Galectin 3 recombinant monoclonal antibody (Cat # RAB02502). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — LGALS3
Entrez GeneID
3958Protein Accession#
P17931Gene Name
LGALS3
Gene Alias
CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2
Gene Description
lectin, galactoside-binding, soluble, 3
Omim ID
153619Gene Ontology
HyperlinkGene Summary
lectin
Other Designations
IgE-binding protein|MAC-2 antigen|carbohydrate-binding protein 35|galactose-specific lectin 3|galectin 3|galectin-3 internal|laminin-binding protein
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Interactome
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Disease
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