LGALS3 recombinant monoclonal antibody, clone 4E1
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: NIH/3T3 whole cell lysate; Lane 4: Colo320 whole cell lysate; Lane 5: HT29 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of LGALS3 recombinant monoclonal antibody, clone 4E1 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of MCF-7 Cells with LGALS3 recombinant monoclonal antibody, clone 4E1 at 1:50, counter-stained with DAPI.
Immunoprecipitation
Immunoprecipitating LGALS3 in Hela whole cell lysate.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LGALS3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human LGALS3.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: NIH/3T3 whole cell lysate; Lane 4: Colo320 whole cell lysate; Lane 5: HT29 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of LGALS3 recombinant monoclonal antibody, clone 4E1 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of MCF-7 Cells with LGALS3 recombinant monoclonal antibody, clone 4E1 at 1:50, counter-stained with DAPI.Immunoprecipitation
Immunoprecipitating LGALS3 in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LGALS3
Entrez GeneID
3958Protein Accession#
P17931Gene Name
LGALS3
Gene Alias
CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2
Gene Description
lectin, galactoside-binding, soluble, 3
Omim ID
153619Gene Ontology
HyperlinkGene Summary
lectin
Other Designations
IgE-binding protein|MAC-2 antigen|carbohydrate-binding protein 35|galactose-specific lectin 3|galectin 3|galectin-3 internal|laminin-binding protein
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Interactome
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Disease
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