TRIM28 recombinant monoclonal antibody, clone R07-5E6
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunocytochemistry
Immunocytochemical staining of Hela with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152). (A) TRIM28 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TRIM28.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human TRIM28.
Theoretical MW (kDa)
Calculated MW: 89 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with TRIM28 recombinant monoclonal antibody, clone R07-5E6 (Cat # RAB02152). (A) TRIM28 (green) and (B) DAPI (blue).Immunofluorescence
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Gene Info — TRIM28
Entrez GeneID
10155Protein Accession#
Q13263Gene Name
TRIM28
Gene Alias
FLJ29029, KAP1, RNF96, TF1B, TIF1B
Gene Description
tripartite motif-containing 28
Omim ID
601742Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq
Other Designations
KRAB-associated protein 1|nuclear corepressor KAP-1|transcriptional intermediary factor 1-beta|tripartite motif-containing 28 protein
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Interactome
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