ATP5F1B recombinant monoclonal antibody, clone R01-4G1
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More Files
- More Functions
Western Blot
Western blot analysis of ATPB in Jurkat, Hela, COS7, Raji lysates using human ATPB recombinant monoclonal antibody, clone R01-4G1 (Cat # RAB01577).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with ATPB recombinant monoclonal antibody, clone R01-4G1 (Cat # RAB01577).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human ATPB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATPB
Theoretical MW (kDa)
Calculated MW: 57 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ATPB in Jurkat, Hela, COS7, Raji lysates using human ATPB recombinant monoclonal antibody, clone R01-4G1 (Cat # RAB01577).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with ATPB recombinant monoclonal antibody, clone R01-4G1 (Cat # RAB01577).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunoprecipitation
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Gene Info — ATP5B
Entrez GeneID
506Protein Accession#
P06576Gene Name
ATP5B
Gene Alias
ATPMB, ATPSB, MGC5231
Gene Description
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Omim ID
102910Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq
Other Designations
ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit|mitochondrial ATP synthetase, beta subunit
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Interactome
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Pathway
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Disease
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